Mammalian Cell Culture

Mammalian Cell Culture

5 day lab & lecture course for industry professionals

Mammalian cell biotechnology has undergone explosive growth over the last 25 years. Recombinant Chinese hamster ovary (CHO) cells now far surpass E Coli and all other expression systems for recombinant protein production. With production levels up 10,000-fold during the same period, recombinant protein production levels are now often measured in grams per liter - and are expected to continue to increase.

Not only the most common system for production of marketed recombinant proteins, mammalian cell culture is also the most used production system for proteins undergoing clinical testing.

This five day intensive is designed to help industry professionals become more knowledgeable, operationally adept, and business savvy. The primary goal of this course is to provide an advanced background in mammalian cell biotechnology.

Academic lead for the course is Rathmann Professor and Director of the Amgen Bioprocessing Center, Matt Croughan, PhD.

Course overview

Scientific, engineering, and practical industrial aspects will be presented in a series of lectures and laboratory exercises in the Amgen Bioprocessing Center at KGI. The laboratory exercises will provide hands-on experience with many of the techniques and principles taught in that day's lectures. Course attendees will gain first hand experience in setting up, running, and analyzing data from high-density, fed-batch cultures in lab scale Applikon bioreactors.

Format

The five day Intensive integrates lectures and projects in a classroom setting with lab work or advanced projects gauged to the experience level of each participant. In general, lectures and projects will occur in the morning, with lab and advanced projects scheduled for the afternoon. This format maximizes content appropriateness for each participant, offers the opportunity to apply lecture material to a lab or project/computational experiment, and provides ample time for student-instructor interaction.

Lecture topics

Mammalian Cell Culture Intensive
  • history and status of mammalian cell biotechnology, including past, current and future products, as well as challenges faced
  • biology of cultured cells, including cell growth, death, metabolism, mortality, transformation, differentiation, and adaptation
  • cell culture kinetics and modeling
  • cell line engineering techniques and common host cell lines for recombinant protein and vaccine production
  • design of cell culture medium and control of cell metabolism
  • cell culture bioreactor design and scale-up, including aeration, mixing, and hydrodynamic/sparging impacts on cells, plus instrumentation and process control
  • design and operation of high-density fed-batch and perfusion cultures
  • integration of cell culture processes with downstream product recovery and purification
  • facility design and operations, from disposables to large-scale stainless steel, including cleaning and sterilization
  • vaccine process development and manufacturing
  • key issues and tools used in industrial cell culture operations, including economies of scale, control charts, and process flow diagrams

Laboratory exercises

  • culture of cells using proper aseptic technique · measurement of cell growth, death, and primary metabolism
  • performance of mammalian cell culture operations under current Good Manufacturing Practices (cGMPs)
  • use of Standard Operating Procedures (SOPs) and Master Batch Records (MBRs)
  • cleaning, sterilization, set-up, and operation of a laboratory-scale bioreactor, for growth of a high-density, fed-batch suspension culture
  • measurement, tracking, and control of culture pH, oxygen, temperature and other environmental parameters
  • thorough analysis of experimental cell culture results, including graphic data presentation, and proper results interpretation with appropriate statistical analyses

Instructors Mammalian Cell Culture Intensive

  • Academic lead for the course is Rathmann Professor and Director of the Amgen Bioprocessing Center, Matt Croughan, PhD.
  • Assisting Dr. Croughan will be Bjorn Ken Lydersen, Ph.D., editor of the best-selling book "Biorprocess Engineering" and a popular lecturer at KGI.
  • Lab instructors will include Drs. Croughan and Lydersen, as well as Kirilynn Svay, Senior Bioprocess Engineer at KGI, and KGI doctoral students in bioprocessing.

Who should apply?

KGI has designed this MCC course for a broad range of industry skills and experiences. The class will provide practical operations training for industry professionals with little/no lab experience as well as for those with considerable bioprocessing background.

Although not required, a rudimentary understanding of microbial or cell culture processes is helpful.

Location

The MCC course will be held at Keck Graduate Institute of Applied Life Sciences, 535 Watson Drive, Claremont CA 91711. KGI is approximately 12 miles from the Ontario, California airport

Fee

The fee is $3999 per attendee. Included within the fee are all lab equipment and supplies, handouts and materials, instruction, daily breakfasts and lunches. Dinners on Sunday and Thursday evenings are also part of the fee. Accommodations and travel expenses are the participant's responsibility. We can assist in securing convenient accommodations in Claremont.

Future dates for this program have not been set; please contact us for additional information.

For more information:
Diana Bartlett, Assistant Vice President, Director of Corporate Partnerships
Keck Graduate Institute
Diana.bartlett[at symbol]kgi.edu
909 607 9864

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