Institutional Laboratory Support
In addition to each faculty member's individual research space and instrumentation, KGI's Institutional Laboratory Support (ILS) provides core laboratory space, instrumentation, and services essential to nurturing interdisciplinary, applied research. Instrumentation within the ILS is accessible to all researchers working at KGI. Instrument maintenance and training is provided through laboratory core personnel. Laboratory oversight, equipment maintenance and user training is provided through the director of the ILS or Jose Salazar Research Associate.
For further information, please contact the Director of the ILS: Barbara Erwin.
The ILS laboratory facilities encompass a 1,866 sq. ft. core laboratory organized into thematic areas, a 316 sq. ft. design and fabrication facility, a 384 sq. ft. Laboratory Automation Center, plus sections of other laboratories at KGI, a small BSL2 lab, a cell culture lab with a laminar flow hood, a class 10,000 soft-walled clean room (Clean Air Products), two utility rooms with autoclaves, dishwashers, drying ovens, freezers and refrigerators, and a dark room with revolving door.
- PCR Instrumentation
- Spectrophotometers and Microplate Readers
- Imaging Systems
- Flow-based Cell Analysis
- DNA Sequencing
- Liquid Handling
- Design & Fabrication
- Material Characterization
- Process Control
- General Instrumentation and Laboratory Infrastructure
Real-Time PCR: Roche Light Cycler (carousel), MJ Research Opticon 1 (96-well plate), Bio-Rad Opticon (96-well plate)
Thermocycler: MJ Research PTC-200 Thermocycler w/ interchangeable heads (96/384 well plates), TechGene 96- well plate Thermocycler.
UV/Vis: Shimadzu UV 1601 Dual beam, Beckman Coulter DU 640 and DU 640i, Tecan Nanoquant M200 (small sample volume), Molecular Devices SpectraMax 384Plus (96 and 384 well plates)
Molecular Devices SpectraMax Gemini EM (96 well plates and various tissue culture plates, top and bottom read).
UVP BioSpec AC Imaging System, Bio-Rad Microplate Imaging System (Ultramark EX model), Transilluminator (Fisher Scientific), 2 UVP Epi Chemi II Darkroom imagers, darkroom with revolving door
Fluorescence microscopes: Leica SP5 Confocal Microscope, Nikon TE2000 fluorescence inverted microscope, Nikon E800M fluorescence compound microscope, LabSmith SVM340 synchronized video microscope
Regular microscopes: Leica MZ75 stereoscope, Nikon Eclipse TS100 inverted microscope
Digital Imaging: CoolSnap CCD cameras (2), Photron FastCam-512PCI 40K fps digital high speed video camera, Cohu 2200 series camera, National Instrument PCI 1401 frame-grabber
Nanoscale Imaging: Veeco BioScope atomic force microscope (Nanoscope IIIa controller, integrated with a Carl Zeiss Axiovert 200 inverted optical microscope enclosed in a Micro-G acoustic/ vibration isolation table), Hitachi H-600 transmission electron microscope
Beckman Coulter FC500 flow cytometer (part of Center for Biomarker Research), Beckman Coulter Cytomics FC 500 MPL, flow cytometry System with MXP software ( available to all ILS users). Coulter Z2 particle counter
Beckman Coulter CEQ 8000 capillary DNA sequencer
Capillary Electrophoresis: Beckman Coulter MDQ capillary electrophoresis system, Micralyne Microfluidic Toolkit (chip-based capillary electrophoresis), Agilent Bioanalyzer
ProteomeLab PF 2D HPLC system, AKTAprime protein purification system (Amersham Pharmacia Biotech)
Beckman Coulter Biomek FXp Liquid Handler with 8-span, 96-well, and 384-well heads, Beckman Coulter Biomek FX Liquid Handler with two 96 well heads
Roland MDX-40 CNC Mill, Stratasys/Dimension SST768 rapid prototyping machine, Sherline/Flashcut 2000 CNC milling machine and 4400 CNC lathe controlled by a PC running SolidWorks CAD software and VisualMill 4.0CAM software, Roland LPX-600 3D-scanner, Universal 30W CO2 laser cutter, Spectra Physik EMG 102 90 Watt excimer laser capable of using ArF(193nm), KrF(248nm), XeCl(308nm) or XeF(351nm) integrated into Florod laser cutting optics, EXFO Acticure 400 100W UV spot curing system, Sharplan 1100 140W CO2 cutting laser
PWM32 series photoresist spinner, ABM Mask Aligner and Blak-Ray 365nm UV lamp, Cookson Electronics PDS 2010 Parylene Reactor, EMITECH dual head Sputter Coater (with QCM thickness detector), Wesbond gold wire ball bonder, Terra-Universal Plasma-Preen plasma etcher, SPI Plasma Prep II etcher/cleaner, Anatech Hummer II gold sputter coater, Fisher Isotemp 381 Vacuum Oven, Ambios XP-1 Profilometer, Maxtek RQCM quartz crystal microbalance
Keithley 2400 source meter, Keithley 2700 multiplexer, California Instruments 351TL high voltage power supply, Lab Smith HVS 200V high voltage sequencer, Tektronix TDS 224 computer-interfaceable oscilloscopes, Tenma 2MHz sweep function generator,
Instron 5542 computer-controlled tensile and compression testing instrument, Cole-Parmer Surface Tensiometer 20, Cambridge Applied Systems ViscoLab 400 digital viscometer, Fisher AB30 conductivity meter, contact angle goniometer, Malvern Zetasisizer (dynamic light scattering),
LabView-based control station with a variety of computer-controlled pumps, pressure transducers, thermocouples with meters, thermoelectric heating-cooling modules, pH and ion selective electrode meters, and computer-controlled power supplies
Shakers and incubators, waterbaths, centrifuges (desktop and floor models, including ultracentrifuge), lyophilizer, speed vac system, Gel Electrophoresis systems (1D, 2D and IEF), balances, pH meters, sonicators (bath and tip),
Refrigerators and Freezers (-20C and -80C), Autoclaves, icemachines, dishwasher and dryer, water purification systems