Mammalian Cell Culture Intensive
3-day Lab & Lecture Course for Industry Professionals
July 23-25, 2014
Advances in mammalian cell technology over the past three decades, combined with major developments in therapeutic proteins and antibodies, have made the recombinant Chinese Hamster Ovary (CHO) cells the expression of choice in biotech manufacturing.
In traditional mechanically stirred bioreactors, production levels have increased 10,000-fold during the same period and product protein titers are now routinely measured in grams per liter. These achievements are leading to significant cell culture bioprocess sophistication, and, along with emerging single use technologies, are making the future flexible bioprocess manufacturing a distinct possibility.
This three day intensive laboratory based course is designed for professional scientists and engineers who wish to enhance their basic knowledge and training in upstream functional areas including cell culture and primary recovery.
The primary goal of the course is to update the participants' background in mammalian cell biotechnology, from cell line design, development and optimization to bioreactor design, scale up and operation.
The course will be delivered by a team of senior industry and academic experts at the Amgen Bioprocessing Center (ABC) at Keck Graduate Institute in Claremont, California, from July 23-25, 2014.
Scientific, engineering, and practical industrial aspects will be presented in a series of lectures and laboratory exercises in KGI's ABC. The laboratory exercises will provide hands-on experience with many of the techniques and principles taught in that day's lectures. Course attendees will gain first-hand experience in setting up, running, and analyzing data from high-density, fed-batch cultures in lab scale Applikon bioreactors.
The three-day Intensive training course integrates lectures and projects in a classroom setting with lab work or advanced projects gauged to the experience level of each participant.
In general, lectures and projects will occur in the morning, with lab and advanced projects scheduled for the afternoon.
This format maximizes content appropriateness for each participant, offers the opportunity to apply lecture material to a lab or project/computational experiment, and provides ample time for student-instructor interaction.
- biology of cultured cells, including cell growth, death, metabolism, mortality, transformation, differentiation, and adaptation
- cell culture kinetics and modeling
- cell line engineering techniques and common host cell lines for recombinant protein and vaccine production
- design of cell culture medium and control of cell metabolism
- cell culture bioreactor design and scale-up, including aeration, mixing, and hydrodynamic/sparging impacts on cells, plus instrumentation and process control
- design and operation of high-density fed-batch and perfusion cultures
- design and operation of single use bioreactors
- integration of cell culture processes with downstream product recovery and purification
- facility design and operations, from disposables to large-scale stainless steel, including cleaning and sterilization
- key issues and tools used in industrial cell culture operations, including economies of scale, control charts, and process flow diagrams Laboratory exercises
- culture of cells using proper aseptic technique
- measurement of cell growth, death, and primary metabolism
- performance of mammalian cell culture operations under current Good Manufacturing Practices (cGMPs)
- use of Standard Operating Procedures (SOPs) and Master Batch Records (MBRs)
- cleaning, sterilization, set-up, and operation of a laboratory-scale bioreactor, for growth of a high-density, fed-batch suspension culture
- measurement, tracking, and control of culture pH, oxygen, temperature and other environmental parameters
- thorough analysis of experimental cell culture results, including graphic data presentation, and proper results interpretation with appropriate statistical analyses
- Parviz Shamlou, PhD, with over 30 years of industry and academic experience in whole bioprocess design and development, will be leading the lectures on engineering design and modeling of cell culture bioreactors including traditional stirred bioreactors and single use bioreactors.
Also lecturing will be:
- Ashraf Amanullah, Ph.D., Senior Director at Gilead, a world leader in bioreactor engineering, including scale-up and scale-down.
- Mike Hilgert, a veteran in bioprocess design, development an manufacturing will lead the section of the course on design, scale up of in the primary recovery including centrifugation, depth and membrane filtration.
- Lab sessions will be led by Kirilynn Svay, Lecturer and Director of Bioprocess Engineering Operations.
Who should apply?
KGI has designed this MCC course for a broad range of industry skills and experiences. The class will provide practical operations training for industry professionals with little/no lab experience as well as for those with considerable bioprocessing background.
The MCC course will be held at Keck Graduate Institute School of Applied Life Sciences, 535 Watson Drive, Claremont CA 91711. KGI is approximately 12 miles from the Ontario, California airport
The fee is $1500 per attendee. Included within the fee are all lab equipment and supplies, handouts and materials, instruction, daily lunches. A welcoming evening reception and Thursday evening dinner are also part of the fee. Accommodations and travel expenses are the participant's responsibility. We can assist in securing convenient accommodations in Claremont.
Requests for refunds due to cancellation for any reason must be made in writing (email: aa.events[at symbol]kgi.edu).
Requests for refunds received by us before Friday, July 11, 2014 will be made as follows:
$2,999 minus $100 processing fee
$3,999 minus $130 processing fee
$4,999 minus $160 processing fee
Requests for refunds received by us between July 14 - 17, 2014, will be made as follows:
$2,999 minus $300 processing fee
$3,999 minus $400 processing fee
$4,999 minus $500 processing fee
No refunds will be given for any reason starting July 18, 2014.