Protein Expression Workshop
Hands-On training at Keck Graduate Institute — learn to make recombinant proteins with the yeast, Pichia pastoris.
Led by Dr. Jim Cregg
This course is designed for researchers from industry and academia who are interested in practical approaches to the development and use of Pichia pastoris for manufacturing a wide spectrum of proteins.
Course participants will gain theoretical and practical experience and skills in the design of an appropriate expression strategy for a target protein (TP). They will learn how to choose the right Pichia pastoris host/vector system, how to select strains to express sufficient TP levels, how to improve target protein expression using best laboratory gene-engineering and technological practice.
During the workshop, participants will learn step-by-step methods for development of a Pichia pastoris production strain, including information on sources of basic materials (strains and vectors), as well as systems for improved protein features, like the "GlycoSwitch" system for "humanization" of glycoproteins and others.
Additionally, students will learn to manipulate vectors and DNA fragments, to introduce an expression cassette into the host, plus methods for selecting and analyzing the resulting recombinant strains.
Also the basic knowledge of small scale expression approaches, appropriate analytical tools, bioprocessing with high cell density fermentation and TP purification will be covered.
Who should attend?
Industrial and academic researchers and students with any background in microbiology, biochemistry, molecular biology or biotechnology will benefit from participation in this interactive workshop.
For more information, contact Diana Bartlett, diana.bartlett[at symbol]kgi.edu, (909) 607 9864.
|Where:||KGI Main Campus|
|When:||February 27, 2014 - March 2, 2014|