A Portable Inexpensive System for Tuberculosis Diagnosis Using Isothermal DNA Amplification Coupled to Lateral Flow Detection
Kristina Roskos, PhD Candidate, is a member of the Niemz Lab team which has been working to develop a system composed of a mesofluidic pouch-based cartridge that mates with a handheld heater, which isothermally amplifies DNA and detects the amplicons via nucleic acid lateral flow (NALF). The closed-system disposable cartridge prevents amplicon carryover. Fluid handling in the cartridge involves one-way passive valves and electrolysis-driven pumping that can be readily integrated into a portable, inexpensive device. Cartridge manufacturing is based on standard low cost and scalable techniques, such as injection molding. Nucleic acid amplification occurs in a flexible plastic film pouch that enables efficient heat transfer and simplified pumping. Isothermal DNA amplification was performed via the exponential amplification reaction (EXPAR) and the loop-mediated amplification (LAMP) reaction, targeting Mycobacterium tuberculosis (M.tb). Using these reactions with NALF detection integrated into the cartridge, the Niemz Lab team has demonstrated detection of M.tb genomic DNA in approximately 60 minutes. A refined version of this amplification and detection cartridge integrated with upstream sample preparation could enable fully automated sample-in to answer-out infectious diseases diagnosis at the point of care in low-resource high-burden countries.
Lunch will be provided.
Please RSVP no later than October 10 to Lindsay Janssen.
|Where:||KGI Main Campus, 152 Classroom|
|When:||October 12, 2011, 12:00 p.m. - 1:00 p.m.|