Course Detail

The synthesis of desired products in microorganisms or plants that do not normally produce these substances relies on the ability to manipulate the relevant DNA sequences. Since the 1970s, genes have been excised, inserted, amplified and expressed using what have now become standard techniques for molecular biologists. Examples of applications of this technology include the production of human insulin which was approved by the FDA in 1982, and genetically modified foods.

This optional laboratory techniques course is intended for students who are new to the biosciences and will provide an understanding of the theory and practice of basic technologies involved in the manipulation of DNA and proteins. The course will cover general laboratory procedures, equipment use and safety considerations and is made up of a series of projects that highlight the most important laboratory techniques currently in use by molecular biologists. Laboratory sessions will build on lectures presented in ALS 300 Molecular Biotechnology.

• Introduction: general lab procedures, equipment use and safety considerations.
• PCR and cloning one of two genes, either human serum albumin (HSA)  or bacterial beta lactamase (Bla)
• Purification of plasmid DNA from cell cultures.
• Restriction enzyme digestion of plasmid DNAs and analysis by agarose gel electrophoresis and UVP analyzer.
• Electroporation
• Gene expression studies to test for synthesis of recombinant protein: SDS PAGE and enzyme activity assays

In taking the laboratory techniques course, students will

1. obtain 'hands on' experience in performing basic molecular biology techniques, including developing protocols and designing experimental strategies.
2. be able to explain the theory underlying each lab technique and identify common applications of each method in biological research.
3. gain experience working in small groups to solve problems.

Laboratory performance 35%
Laboratory Quiz 35%
Laboratory Report or Poster Presentation 30%