James M. Cregg,
PhD
ProfessorAreas of Expertise:
- Recombinant Protein Expression
- Peroxisome Biogenesis
- Molecular Biology
- Pichia pastoris
James M. Cregg teaches courses on the structure and function of genes and recombinant gene expression. Dr. Cregg’s research focus led to the development and use of a yeast, Pichia pastoris, as a system for the production of recombinant proteins. His work has had a major impact on the development of pharmaceutical proteins such as a vaccine against hepatitis B; human serum albumen, a component for an artificial blood plasma; and the anti-angiogenic proteins, angiostatin and endostatin that have garnered attention for their potential anti-cancer properties. He holds 14 patents in this area. Dr. Cregg earned his PhD in biology from
Rice University. Previous select appointments have been with the Oregon Graduate Institute, the Salk Institute Biotechnology Industrial Associates, and BRL/Life Technologies.
Last updated 12.14.2006
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| O. Cos, A. Serrano, J.L. Montesinos, P. Ferrer, J.M. Cregg, and F. Valero, (2005) Combined effect of the methanol utilization (Mut) phenotype and gene dosage on recombinant protein production in Pichia pastoris fed-batch cultures, J. Biotech. 116, 321-335.
D. Esposito, W. K. Gillette, D. A. Miller, T. E. Taylor, P. H. Frank, R. Hu, J. Bekisz, J. Hernandez, J.M. Cregg, K.C. Zoon, and J.L. Hartley, (2005) Gateway cloning is compatible with protein secretion from Pichia pastoris, Prot. Exp. Purif. 40, 424-428.
C. Ilgen, J.L. Cereghino, and J.M. Cregg, Chapter 7: Pichia pastoris. In: Production of recombinant proteins: microbial and eukaryotic expression systems. Gellissen, G. (ed.) Wiley-VCH Verlag,
Weinheim, Germany, pp.143-162 (2005).
D. Thor, S. Xiong, C.C. Orazem, A.-C. Kwan, J.M. Cregg, J. Lin-Cereghino, and G.P. Lin-Cereghino, (2005) Cloning and characterization of the Pichia pastoris MET2 gene as a selectable marker. FEMS Yeast Res. 5, 935-942.
O.V. Stasyk, O.G. Stasyk, J. Komduur, M. Veenhuis, J.M. Cregg, and A.A. Sibirny, (2004) A hexose transporter homologue controls glucose repression in the methylotrophic yeast Hansenula polymorpha. J. Biol. Chem. 279, 8116-8125.
A.J. Sunga, and J.M. Cregg, (2004) The Pichia pastoris formaldehyde dehydrogenase gene (FLD1) as a marker for selection of multicopy expression strains of P. pastoris. Gene 330, 39-47. O.V. Stasyk, T.Y. Nazarko, O.G. Stasyk, O.S. Krasovska, D. Warnecke, J.-M. Nicaud, J.M. Cregg, and A.A. Sibirny, (2003) Sterolglucosyltransferases have different functional roles in Pichia pastoris and Yarrowia lipolytica. Cell Biology International 27, 947-952.
D.J. Klionsky, J.M. Cregg, W.A. Dunn, S.D. Emr, Y. Sakai, I.V. Sandoval, A. Sibirny, S. Subramani, M. Thumm, M. Veenhuis, and Y. Ohsumi, (2003) A Unified nomenclature for yeast autophagy-related genes. Developmental Cell 5, 539-545. R.J.S. Baerends, G.J. Sulter, T.W. Jeffries, J.M. Cregg, and M. Veenhuis, (2002) Molecular characterization of the Hansenula polymorpha FLD1 gene encoding formaldehyde dehydrogenase. Yeast 19, 37-42.
G.P. Lin Cereghino, J. Lin Cereghino, C. Ilgen, and J.M. Cregg, (2002) Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris. Curr. Op. Biotech. 13, 329-332.
J.M. Cregg, A. Lapidus (2002) Genomic sequencing of the methylotrophic yeast Pichia pastoris. Mol. Biol. of the Cell 13, 748. |
12.14.2006
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| Candida tropicalis transformation system, patent 5,204,252. Issued April 20, 1993, James M. Cregg, inventor
Transformation of yeasts of the genus pichia, patent 4,879,231. Issued November 7, 1989, James M. Cregg, inventor
Genes from pichia histidine pathway and uses thereof, patent 4,885,242. Issued December 5, 1984, James M. Cregg, inventor
Autonomous replication sequences for yeast strains of the genus pichia, patent 4,837,148 Issued June 6, 1989, James M. Cregg, inventor
Site selective genomic modification of yeast of the genus pichia, patent 4,882,279 Issued November 21, 1989, James M. Cregg, inventor
Pichia pastoris argininosuccinate lyase gene and uses thereof, patent 4,818,700 Issued April 4, 1989, James M. Cregg, inventor
Yeast production of hepatitis B surface antigen, patent 4,895,800 Issued January 23, 1990 James M. Cregg, inventor
Pichia transformation, patent 4,929,555 Issued May 29, 1990, James M. Cregg, inventor
Pichia pastoris alcohol oxidase II regulatory region, patent 5,032,516 Issued July 16, 1991, James M. Cregg, inventor
Strains of pichia pastoris created by interlocus recombination patent 5,122,465 Issued June 16, 1992, James M. Cregg, inventor
Cultures of yeast of the genus Pichia altered by site selective genomic modification patent 5,135,868 Issued August 4, 1992, James M. Cregg, inventor
DNA encoding the alcohol oxidase 2 gene of yeast of the genus Pichia patent 5,166,329 Issued November 24, 1992, James M. Cregg, inventor
Promoter for the Pichia pastoris formaldehyde dehydrogenase gene FLD1 patent 6,730,499 Issued May 4, 2004, James M. Cregg, inventor
Formaldehyde dehydrogenase genes from methylotrophic yeasts patent 20030215920 Filed November 20, 2003 James M. Cregg, inventor |
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 Contact InformationJames M. Cregg
909/607-8562
James_Cregg@kgi.edu
ResourcesJames Cregg's research site
PEX Gene Database
Heterologous Proteins Expressed in Pichia pastoris
Pichia pastoris strains & vectors available from KGI
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