The purpose of this basic laboratory course is to provide the students with hand on experience in design and construction of the most widely applicable vector designs. There are a large number of variations possible in the design of vectors depending on, for example, the expression system and the uses they are put to. This course focuses on teaching the students the basic approaches and methods used in the laboratory and in a manufacturing. Students will gain knowledge of the use of selectable markers, promoters, vector targeting and linearization and analytical methods such as southern blot analyses.
MEng program students only