This course is designed to offer students a comprehensive and practical understanding of the fundamentals of upstream processing. Through hands-on experience, students will gain valuable insights into various aspects of this field. The curriculum covers a wide range of topics, including growth studies conducted in shake flasks for both bacterial and mammalian cells, performing assays to measure growth and enzyme kinetics, as well as designing laboratory protocols. One of the key components of the course is conducting experiments that enable students to compare and contrast microbial strains with mammalian cells. By exploring these different systems, students will have the opportunity to analyze and evaluate their characteristics, advantages, and limitations. Students will keep accurate detailed experimental plans, lab notebooks, batch records, analysis results and experimental reports. Furthermore, teamwork and collaboration will be emphasized in this course. Students will be organized into teams and given the opportunity to present their findings through oral presentations. These presentations will serve as a platform for sharing results and engaging in thoughtful group discussions.