This laboratory course provides extensive hands-on experience in the KGI’s lab-scale facility for mammalian cell culture in conjunction with the theoretical course ENG 5130 Mammalian Cell Culture Technology. Students will learn how to work in a cell culture lab properly including essential aseptic skills, media preparation, cell handling and processing, autoclaving, bioreactor assembly and operation, sensor calibration and monitoring, and use of analytical equipment. They will manage to work collaboratively as a team for their cell culture laboratory projects. They will process the data generated in the lab and present their results through PowerPoint slides and a written report. Through these laboratory cell culture studies, students will enhance their understanding of mammalian cell culture in the shake flasks and bioreactors.