This course is a companion to the Human Genomics lecture and literature-based course GENE 5250. In this lab, students will prepare genomic DNA extractions from human cells and quantify the amount and quality of nucleic acid preparations. Students will identify variants within DNA sequencing reads. Students will carry out an RNA-seq experiment and perform the downstream data analysis pipeline to identify differentially expressed genes. Lastly, students will conduct a human genome sequencing project, including sequence alignment and variant calling. In addition to laboratory skills, students will gain experience in sample documentation, pipeline documentation, and scientific report writing.