This course is a companion to the Human Genomics lecture and literature based course GENE 360. In this lab, students will prepare genomic DNA and RNA extractions from human cells and quantify the amount and quality of nucleic acid preparations. Students will use pyrosequencing to determine the sequence of a small genomic region and identify variants within the sample set. Students will design and carry out a RNA-seq experiment and perform the downstream data analysis pipeline to identify differentially expressed genes. Results will be validated with qPCR. Lastly, students will conduct a whole human genome sequencing project, including sequence alignment and variant calling. In addition to laboratory skills, students will gain experience in sample documentation, pipeline documentation, and scientific report writing.
GENE 360 or concurrent registration