Assessing the Influence of Sequence Duplications and Genome Annotations on Transcriptional Profiling Measurements - Keck Graduate Institute May 3, 2024, 12:00 pm Skip to main content
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Rodolfo Aramayo
Rodolfo Aramayo
Associate Professor, Department of Biology Texas A&M University

The rapid advancement of Next Generation DNA/RNA and Single Cell Sequencing Technologies is fundamentally reshaping the landscape of Biology. However, the main complexity lies in the processing of this data. For instance, RNA sequencing (RNA-seq), a widely adopted method for discerning gene expression variations between two conditions, can yield divergent results from identical datasets. This complexity further escalates when considering the source of Genome Annotation files employed in the analysis. In this discourse, I will elucidate the pitfalls inherent in current RNA-seq analysis methodologies and propose refinements to RNA-seq pipelines aimed at enhancing the accuracy, specificity, and rigor of studies on differential gene expression. Our endeavor underscores the pressing necessity for algorithmic innovations capable of evaluating the contribution of each transcript isoform to overall gene expression levels.

Zoom: https://kgi.zoom.us/j/94707393644

Details

Date:
May 3
Time:
12:00 pm - 1:00 pm PDT
Event Category:

Venue

KGI 121 Building – Room 1111
121 Bucknell Ave.
Claremont, 91711 United States

Other

Open To
General Public, Faculty and Staff, KGI Students