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Please join us for Jonas Otoo’s PhD Dissertation Defense on:
Nucleic acid amplification tests (NAATs) have relatively high sensitivity and specificity. However, they are more complex to develop than other diagnostic tests, such as lateral flow immunoassay tests. Polymerase chain reaction (PCR) is the gold standard of NAATs. PCR requires thermal cycling to achieve clonal amplification of the target DNA for diagnosis. Thermal cycling poses a challenge in the development of PCR diagnostics for point-of-care (POC) settings. Loop-mediated isothermal amplification (LAMP) offers an isothermal method for NAATs diagnostics. The advancement of the microfluidics field significantly enhances the development of LAMP diagnostics devices for POC testing. We have developed an assay for executing droplet microfluidics LAMP via a microparticle primer payload mechanism and have demonstrated it with urinary tract infection (UTI) pathogens. With inspiration from overhang PCR and RNA-Seq, we engineered LAMP primers with 5’ poly-Thiamine (PolyT) oligonucleotide. The PolyT sequence is recognized by a biotinylated capture oligonucleotide engineered with a polyadenylated (PolyA) polynucleotide on the 3’ end. The streptavidin-coated microparticles functionalized with the PolyA oligonucleotide and PolyT primers, capture their specific target DNA and deliver the cargo into emulsion droplets of LAMP reagents for amplification.
Date: Friday, April 21, 2023
Time: 1 – 3 p.m.
Location: 121 Bldg. – Room 1111