Research Seminar Speaker Series: Jaehwan Kim, M.S. – “Microfluidic Auto-sampler for Raman Spectroscopy in Cell Therapy Manufacturing”

Please join us for our Research Seminar Speaker Series with Jaehwan Kim, M.S., MIT Department of Electrical Engineering and Computer Science, PhD Candidate

The ability to readily monitor metabolite concentrations in cell culture media could enable tighter culture condition control and potential improvements in yield and clinical potency of a cell therapy product. In this work, a microfluidic autosampler for in vitro measurements of glucose using Raman spectroscopy for bioprocessing applications is demonstrated. The platform aseptically delivers samples from multiple input vessels to a microfluidic optical flow cell. Discrete sample plugs prevent cross-talk between measurements and minimize dead volume. Raman spectroscopy enables glucose measurements without reagent consumption with a linear range from 0.5 to 4mg/mL. Automated measurements of glucose levels in mesenchymal stromal cell (MSC) culture media demonstrate a limit-of-detection (LOD) of 0.58 mg/mL – sufficient for MSC and other cell culture glucose monitoring.

Date:  Friday, December 3, 2021

Time:  12:00 – 1:00 p.m.

Via Zoom:  https://kgi.zoom.us/j/94499690112