Ilya Tolstorukov, PhD, DSc

2001–Present: Research Professor, Senior Research Scientist, Visiting Scientist, Riggs School, KGI.

2008–Present: Founder, Principal Scientist, Biogrammatics, Inc, Carlsbad, CA.

1991–2002: Section Head, Professor, Section for Genetics of Methylotrophic Yeasts, State Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, Russia.

1997–1998: Visiting Professor, International Center of Biotechnology, Department of Biotechnology, Faculty of Engineering, Osaka University, Osaka, Japan.

1995: Visiting Scientist, Laboratory for Electron Microscopy, Biological Centre, University of Groningen, Haren, The Netherlands.

1989–1990: Visiting Scientist, Laboratory of Eukaryotic Gene Expression, Frederick Cancer Research Facility, National Cancer Research Institute, Frederick, MD, USA

1969-1991: Junior Research Scientist, Senior Research Scientist, Leading Scientist, Group Leader, All-Union Research Institute of Genetics and Selection of Industrial Microorganisms (VNII Genetika), Moscow, USSR

1. G.Naumov, I.Tolstorukov. 1973. Comparative genetics of yeasts. Communication 10. Re-identification of mutators of mating types in Saccharomyces. Soviet Genetics, v.9, No.1, 82-91.
2. I.Tolstorukov, T.Dutova, S.Benevolensky, Ya.Soom. 1977. Hybridization and genetic analysis of methanol-utilizing yeast Pichia pinus. Soviet Genetics, v.13, N.2, 322-329.
3. I.Tolstorukov, S.Benevolensky. 1982. Genetic control of cell type and complex organization of the mating type locus in the yeast Pichia pinus. Current Genetics, 5, 137-142.
4. K.Lahthev, Y.Semenova, I.Tolstorukov, I.Van der Klei, M.Veenhuis. 2002. Isolation and properties of genetically defined strains of the methylotrophic yeast Hansenula polymorpha CBS4732. Arch. Microbiol., 177, 150-158.
5. G.Lin-Cereghino, L.Godfrey et al. 2006. Mxr1p: a key regulator of the methanol-utilization pathway and peroxisomal genes in Pichia pastoris. Mol. and Cell Biol., V.26, No. 3, 883-897.
6. A.J.Sunga, I.Tolstorukov and J.M.Cregg. 2008. Post-transformational vector amplification in the yeast Pichia pastoris. FEMS Yeast Res. 8, 870-876.
7. J.M.Cregg, I.I.Tolstorukov, A.Kusari, J.Sunga, K.Madden, T.Chappell. 2009. Expression in the yeast Pichia pastoris. Review. Methods Enzymol. 463, 169-189.
8. T.M. Ferguson, K.M. Weigel et al. 2016. Pilot study of a rapid and minimally instrumented sputum sample preparation method for molecular diagnosis of tuberculosis. Sci Rep. 20, 6:19541. doi: 10.1038/srep19541.
9. F. Cerini, H. Gaertner et al. 2016. A scalable low-cost cGMP process for clinical grade production of the HIV inhibitor 5P12-RANTES in Pichia pastoris. Protein Expr Purif. 119:1-10. doi: 10.1016/j.pep.2015.10.011.
10. L. Sturmberger, T.G. Chappell, et al. 2016. Refined Pichia pastoris reference sequence. J Biotechnol. S0168-1656(16)30203-6. doi: 10.1016/j.jbiotec.2016.04.023.

1. I.Tolstorukov, T.Prosvirova, et al. 1993. Strain of Candida utilis for production of single cell protein. Certificate of Invention (Russia), RU N 93019585.
2. J.Cregg, J.Latham, M.Litton, R.Schatzman, I.Tolstorukov. 2011. Methods of synthesizing heteromultimeric polypeptides in yeast using a haploid mating strategy. US Patents 7927863, 8268582 and 8709756.
3. J.Cregg, I.Tolstorukov. 2012. P. pastoris ADH promoter and use thereof to direct expression of proteins. US patent 8222386.
4. J.Cregg, I.Tolstorukov. 2014. Novel P. pastoris promoters, and the use thereof to direct expression of proteins in yeast, preferably using a haploid mating strategy. US Patent 8785613.