Jim Cregg, PhD

Dr. Cregg earned his PhD in biology from Rice University in Houston, TX in 1978 and was a postdoctoral fellow at Scripps Clinic and Research Foundation in La Jolla, CA from 1978 to1980. Previous appointments have been with BRL/Life Technologies in Gaithersburg, MD (1980-1983), the Salk Institute Biotechnology Industrial Associates in San Diego, CA (1983-1989), and the Oregon Graduate Institute in Beaverton, OR (1989-1999). In 1988, Dr. Cregg also spent a year on sabbatical in the Netherlands at the State University in Groningen.

Dr. Cregg was one of the founding Professors at KGI and has continued his research on the development and use of yeast, Pichia pastoris, as a system for the production of recombinant proteins, for which he is internationally known. His work has majorly impacted the development of pharmaceutical proteins such as the chimeric monoclonal antibody, which works against rheumatoid arthritis and certain cancers, and human serum albumen, a component of an artificial blood plasma; he holds 14 patents in this area. Dr. Cregg has been invited to give keynote addresses at numerous meetings and serves as a reviewer or executive editor on five peer-reviewed journals in the biotechnology field.

Ternes P, Wobbe T, Schwarz M, Albrecht S, Feussner K, Riezman I, Cregg JM, Heinz E, Reizman H, Feussner I, et al. “Two pathways of sphingolipid biosynthesis are separated in the yeast Pichia pastoris”. Biological Chemistry 2011;286(13):11401-11414.

Nazarko VY, Nazarko TY, Farre JC, Stasyk OV, Warnecke D, Ulaszewski S, Cregg JM, Sibirny A, Subramani S. “Atg35, a micropexophagy-specific protein that regulates micropexophagic apparatus formation in Pichia pastoris”. Autophagy 2011;7(4):375-385.

Zhang P, Zhang W, Zhou X, Bai P, Cregg JM, Zhang Y. “Role of two Pichia pastoris hexose transporter homologues in sugar uptake and catabolite derepression (sugar-induction) of AOX1 promoter”. Applied and Environmental Microbiology 2010;76:6108-6118.

Cregg JM, Tolstorukov I, Kusari A, Sunga J, Madden K, Chappell T. “Expression in the Yeast Pichia Pastoris”. Guide to Protein Purification, Second Edition 2009;463:169-189.

Wriessnegger T, Sunga AJ, Cregg JM, Daum G. “Identification of phosphatidylserine decarboxylases 1 and 2 from Pichia pastoris”. Fems Yeast Research 2009;9(6):911-922.

Sunga AJ, Tolstorukov I, Cregg JM. “Posttrans-formational vector amplification in the yeast Pichia pastoris”. Fems Yeast Research 2008;8(6):870-876.

Hartner FS, Ruth C, Langenegger D, Johnson SN, Hyka P, Lin-Cereghino GP, Lin-Cereghino J, Kovar K, Cregg JM, Glieder A. “Promoter library designed for fine-tuned gene expression in Pichia pastoris”. Nucleic Acids Research 2008;36(12):e76.

Wriessnegger T, Gubitz G, Leitner E, Ingolic E, Cregg J, de la Cruz BJ, Daum G. “Lipid composition of peroxisomes from the yeast Pichia pastoris grown on different carbon sources”. Biochimica et Biophysica Acta-Molecular and Cell Biology of Lipids 2007;1771(4):455-461.

Klionsky DJ, Abeliovich H, Agostinis P, Agrawal DK, Aliev G, Askew DS, Baba M, Baehrecke EH, Bahr BA, Ballabio A, et al. “Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes”. Autophagy 2008;4(2):151-175.

Cregg JM (Ed.) Methods in Molecular Biology. 2nd. 2007. Totowa, NJ., Humana Press. Pichia Protocols.

Cregg JM. “Introduction: Distinction Between Pichia pastoris and Other Expression Systems”. In: Cregg JM, editor. Methods in Molecular Biology. 2nd ed. Totowa, NJ.: Humana Press; 2007;389:1-10.

Cregg JM. “DNA-Mediated Transformation”. In: Cregg JM, editor. Methods in Molecuar Biology. 2nd ed. Totowa, NJ.: Humana Press; 2007;389:27-42.

Tolstorukov I, Cregg, JM. “Classical Genetics”. In: Cregg JM, editor. Methods in Molecular Biology. Totowa NJ.: Humana Press; 2007;389:189-201.

Leon S, Zhang L, McDonald WH, Yates J, Cregg JM, Subramani S. “Dynamics of the peroxisomal import cycle of PpPex20p: ubiquitin-dependent localization and regulation”. Journal of Cell Biology 2006;172(1):67-78.

Stasyk OV, Stasyk OG, Mathewson RD, Farre JC, Nazarko VY, Krasovska OS, Subramani S, Cregg JM, Sibirny AA. “Atg28, a novel coiled-coil protein involved in autophagic degradation of peroxisomes in the methylotrophic yeast Pichia pastoris”. Autophagy 2006;2(1):30-8.

Cregg JM, Gleeson MAG, Picattaggio S, Haas LOC, inventors; Henkel Research Corporation, assignee. Candida tropicalis transformation system. Santa Rosa, CA / United States patent 5,204,252. 1993 Apr 20.

Cregg JM, inventor; Research Corporation Technologies I, assignee. Formaldehyde dehydrogenase genes from methylotrophic yeasts. Arizona / United States patent 7,745,200. 2010 Jun 29.

Cregg JM, Tolstorukov I, Latham J, Litton MJ, Schatzman R. Methods of synthesizing heteromultimeric polypeptides in yeast using a haploid mating strategy. PCT/US2004/035302.