This course will provide in-depth and hands-on training in using genome-wide data to answer functional questions in biology. Students will gain experience in gene expression studies using polyA-enriched RNA sequencing and data analysis to identify differentially expressed genes in multiple samples. Other techniques of RNA sequencing will be discussed, including miRNA-seq and nuclear run-on sequencing. The second half of the course will explore the interaction between proteins and DNA in the context of ChIP-seq experiments and chromatin conformation capture techniques. The role of chromatin modification in cell function will also be investigated in the context of methylation profiling. The course will culminate in a discussion of discoveries made through major genomics initiatives such as the ENCODE project and the Epigenetics Roadmap.